Journal: Cancers

Article Title: Poliovirus Receptor as a Potential Target in Gastric Signet-Ring Cell Carcinoma for Antibody-Drug Conjugate Development

doi: 10.3390/cancers18020270

Figure Lengend Snippet: Identification of PVR as a GSRCC target. ( A ) Evaluation of PVR expression on human GSRCC cells indicated by FACS; ( B ) IF staining of PVR in MKN45, KATO3 and NUGC4 cells. Scale bar is 100 μm; ( C ) Internalization of PVR Ab by MKN45 and NUGC4 were detected by IF staining within 4 h. Scale bar is 20 μm; ( D ) Cell internalization assay using pHrodo Red-labeled PVR antibodies; ( E ) Line charts showing the internalization of PVR Ab by MKN45 and NUGC4 acquired by FACS; ( F ) Schematic diagram of biodistribution assay strategy; ( G ) Tumor-bearing mice were dissected to observe detailed biodistribution profile in organs containing heart, liver, spleen, lung, kidney and tumor; ( H ) Quantified the biodistribution of IgG-Cy3, PVR-Cy3, in normal organs (heart, liver, spleen, lung, kidney) and tumor. Data are shown as mean ± s.e.m. NS not significant; ( I ) Average fluorescent intensity of IgG-Cy3 and PVR-Cy3 in tumors, Unpaired t -test, *** p < 0.001; ( J ) Ex vivo biodistribution of PVR-MC-GGFG-Cy3 in each organ of heart, liver, spleen, lung, kidney and tumor; ( K ) Quantified the biodistribution of IgG-MC-GGFG-Cy3, PVR-MC-GGFG-Cy3 in normal organs (heart, liver, spleen, lung, kidney) and tumors. Data are expressed as mean ± s.e.m., NS not significant; ( L ) Mean fluorescence intensity of IgG-MC-GGFG-Cy3 and PVR-MC-GGFG-Cy3 in tumors, Unpaired t -test, *** p < 0.001.

Article Snippet: Briefly, 1 mg of PVR antibody was gently mixed with a 3 molar equivalent of Cy3 NHS ester (MedChemExpress, Monmouth Junction, NJ, USA).

Techniques: Expressing, Staining, Labeling, Ex Vivo, Fluorescence